时分复用双光子成像系统高速数据处理研究

Research on High-speed Data Processing of Time-division Multiplexing Two-photon Imaging System

摘要:

为满足高频脉冲时分复用的双独立扫描探测双光子显微成像系统中信号解多路复用、行场同步、像素值重建的需求,对高速数据的采集处理进行了研究；基于 LabVIEW FPGA,结合有限状态机、FIFO缓存和并行流水线等技术,设计了一种多功能采集处理系统,通过精确的时序控制,成功实现了高速数据的无损解复用和后续成像数据处理,并能够对共振振镜非线性运动导致的图像畸变进行校正；经过调试仿真和实验测试表明,在系统单通道采样率800MSPS、200MHz FPGA处理时钟下,能够对160 MHz时间交错激光脉冲产生的来自不同位置的相应荧光信号进行复杂处理；经实际应用表明,系统满足实时成像的需求,为相关领域的研究者提供了有益的参考。

Abstract:

In order to satisfy the needs of signal demultiplexing, line-field synchronization and pixel value reconstruction in a high-frequency pulse time-division multiplexed dual-independent scanning detection two-photon microscopy imaging system, the acquisition and processing of high-speed data were studied; Based on LabVIEW FPGA, combined with finite state machine, FIFO cache and parallel pipeline technologies, a multifunctional acquisition and processing system was designed，which successfully achieved lossless demultiplexing of high-speed data and subsequent imaging data processing through precise timing control, and was able to correct image distortion caused by the nonlinear motion of the resonant galvanometer; after debugging, simulation and experimental testing, Under the system's single-channel sampling rate of 800MSPS and 200MHz FPGA processing clock, it can perform complex processing on the corresponding fluorescence signals from different locations generated by 160 MHz time-staggered laser pulses. Practical applications have shown that the system meets the needs of real-time imaging and provides solutions for related fields. The researchers provided useful references.