Aiming at the problem of multi-channel fault parallel injection in Avionics System integration test of civil aircraft, a fault injection method of ARINC429 Bus is studied and its function is designed. Firstly, the data interaction mode of avionics relay system is analyzed, and the parallel multi-channel fault injection method based on reflective memory communication network is studied, and the fault injection architecture based on avionics relay system is designed. Then, the fault types and corresponding injection methods of ARINC429 Bus in protocol layer and application layer are analyzed, and the logic design of fault injection function is realized. Finally, a fault injection experiment platform is built based on avionics relay system to carry out fault injection experiment. The experimental results show that the fault injection method can effectively complete fault injection of multi device and multi-channel avionics bus with low delay in protocol layer and application layer, and meet the requirements of Avionics System integration test.