For the unsteady vibration signal, spectrum ambiguity will be produced by FFT analysis. In order to eliminate spectral ambiguity, the most commonly used method is to calculate order tracking, which is mainly carried out by commercial software. In this paper, according to the order analysis process, the speed profile is calculated, and the Lagrange 7-point interpolation algorithm is used; the resampling time is calculated by using the given angular acceleration threshold segmentation interpolation method; the angular domain resampling adopts the sliding low-pass filter interpolation method. The order analysis software system is designed based on LabVIEW. Through the analysis of simulation signal and measured signal, the order analysis system based on LabVIEW can effectively solve the spectrum ambiguity phenomenon.