基于FPGA和STM32的流式细胞仪数据采集系统设计

Design of flow cytometry data acquisition system based on FPGA and STM32

摘要:

针对流式细胞仪采集微弱信号高速高精度的需求,设计了一种基于FPGA和STM32控制的模数转换多通道高速高精度数据采集系统。该系统接收并调理放大光电转换后的微伏级细胞脉冲电信号,经模数转换传给FPGA作识别,再由STM32读取传至上位机分析处理。FPGA结合STM32单片机控制其外围芯片,可实现数据的同步采集、实时缓存和高速传输。为验证系统的有效性,采用彩虹八峰微球上机测试,实验结果表明荧光通道荧光检出限以及荧光线性符合国家标准,满足流式细胞仪数据采集的高速高精度要求,在医疗电子仪器设计领域具有广阔的应用前景。

Abstract:

In order to meet the demand of high-speed and high-precision acquisition of weak signal acquisition by flow cytometer, a multi-channel high-speed and high-precision data acquisition system based on FPGA and STM32 control is designed. The system receives and modulates the microvolt-level cell pulse electrical signal after amplification and photoelectric conversion, and transmits it to the FPGA for identification by analog-to-digital conversion, and then reads and transmits it to the upper computer for analysis and processing by STM32. FPGA combined with STM32 MCU is used to control its peripheral chips, it realizes synchronous data acquisition, real-time buffering and high-speed transmission. In order to verify the effectiveness of the system, it was tested by rainbow eight-peak microspheres. The experimental results showed that the detection limit of the fluorescence channel and fluorescence linearity were in line with national standards and met the high precision requirements of flow cytometry data acquisition, which means it has a broad application prospect in the field of medical electronic instrument design.