Aiming at the Real-time performance requirement of ARINC429 bus in military hardware, a ARINC429 real-time communication method is proposed in this paper. This method is established on RTX real-time system. The hardware of this method is AMC5206B with a PXI bus. In order to be embedded into test process, a RTX application program has been designed. The communication bridge between the test process in windows and RTSS process is the shared memory. The driver program under RTX system is designed to improve the efficiency by the interrupt service. And application program under windows system verified the performance of this method.