AUTOSAR is a open software architecture standard in automotive E/E system. On the basis of full study and research of AUTOSAR structure,the CAN Communication Protocol Stack was developed with hierarchy and standardization,also loaded and tested on the micro controller of MC9S12XEP100 .The results prove that the Protocol Stack can greatly realize communication between different layers of CAN,which can meet the requirements of Vehicle bus communication;it is also capable of compatibility and transportation,which can observably increase the code transporting speed between different hardware platforms, decrease the software developing time and also increase the code reliability.