The temperature controller has been important in aircraft temperature conditioner system, which needs to be measured according to its lots of parameters. The traditional test system has some disadvantages of imprecise, bad compatibility and too much working load. The general automatic testing technology of temperature controller is mentioned based on PXI bus. Its testing scheme and measurement is introduced including the hardware and software. The resistance piecewise approximation within a closed-loop and the low latency algorithm of pulse output are also importantly applied. This testing system has the advantages of generality, extensibility and intelligence.