In the past, the tungsten distance couldn’t be precisely measured during the sealing welding process of nuclear fuel rods and real-time observation of internal welding chamber as well as to save the welding images were also unrealized. To solve that, an image acquisition and processing system based on LabVIEW for the plugging holes welding is designed. Unlike traditional projection methods, the system includes two digital cameras to obtain the internal images of the chamber and welding videos. Then analysis the relative position between tungsten and the nuclear fuel rod end plug. And then save the analysis results and the images to the corresponding record. Welding experiments show that the image system can obtain high quality internal image and work stably, and the analysis is accuracy and reliable; According to the experiments results, summarized the suitable tungsten distance.