Abstract:With the continuous development of communication technology and the increasing demand of network usage, the stability and security of network equipment directly related to the quality of Internet technology. According to the "《IEEE 802.3-2000 and ANSI X3.263-1995 standard》" requirements on Ethernet physical layer, to 100Base-TX Network Interface Physical Layer Conformance Test project research, design a network interface Physical Layer Conformance Test method, through test results show that the method is simple and effective, which can meet the requirements related to the physical layer conformance test.