As Cryogenic Power System being used in the design of the new generation launch vehicle, more and higher requirements for reliability、continuous working time、testability、expansibility and interface relationship have been put forward to the design of power supply monitoring subsystem in the Measuring System of new launch vehicle. A new power supply monitoring subsystem design based on 1553B bus is proposed to achieve these requirements. In this article, the overall scheme hardware and software are shown, parts of the key technologies used in the system are discussed:intelligentizing of power equipments, interface design between space launch vehicle and ground-monitoring system, protocol of power supply monitoring subsystem and dual-PXIs redundant warm backup. This new power supply system has been successfully passed all the large-scale test for the new generation launch vehice, and has been promoted in other projects.