In the light of the requirements for remote monitor the local collected data in real time, according to the powerful function module of LabVIEW as well as the flexible development characteristics of Arduino, proposed the Arduino as the controller, using the design idea of interactive programming by Arduino software together with LabVIEW software. In order to slove the limitation of Web publishing by LabVIEW in local server, meanwhile to simplify the hardware design of the system and increase the fiexible of use, with the help of cloud computing as a server, design implements of a wireless serial acquisition and control system based on LabVIEW and Arduino, this system can also realize real-time synchronization Web publishing. The result of the test indicate that this system has characteristics of friendly interface, high reliability and expansibility, at the same time it successfully establish Web request through Webpage on the internet.