磁珠法核酸自动提取温度控制系统设计

Temperature System Design for Automatic Nucleic Acids Extraction Instrument

摘要:

针对自主设计的基于纳米磁珠的自动核酸提取系统设计了温度系统,主要包含系统温度控制算法分析、硬件电路设计及相关软件设计;系统温度采用全加热和在固定频率及占空比PWM波控制下间歇性加热的分段控制,通过实验确定全加热时间及PWM波的占空比,并建立知识库,可以满足使用多种磁珠法核酸提取试剂盒进行核酸提取的温度要求；用该系统为河南惠尔纳米科技公司试剂盒中裂解液及洗脱液加热,实验发现,裂解时8个裂解孔的最大温度差异小于6 ℃,洗脱时8个洗脱孔的最大温度差异小于5 ℃,均满足核酸提取实验时对温度的要求。

Abstract:

The temperature system was designed for an automatic nucleic acids extraction instrument, including temperature control algorithm design, circuit design, and software design. The temperature control mode included two parts, one is full heating stage, the second is PWM heating stage. Numerous experiments shows that the frequency and duty ratio of the PWM are constants according to the production by several companies. The database was built to fix some parameters by different model. Optimize the parameters of temperature control mode, it showed that the maximum temperature difference of lysate and elution respectively is 6℃ and 5℃.