For the requirement of high accuracy and high reliability attitude determination of coming satellites applications, the single-axis spacecrafts attitude determination physical simulation system was designed. For the sun sensors and gyros integrated mode, an attitude determination algorithm based on the extended Kalman filter was proposed. The software was designed by C language. The system was based on DSP, and a multi sensors data interface was designed. The physical Simulation results:attitude determination accuracy of better than 0.5°. The gyro constant drift estimated average of 3.625758e-4°/s, the experimental results show the effectiveness of the algorithm Attitude Determination and application of the technology for the engineering feasibility.